| 1. | Christopher Horner (USA, Team Radioshack) | 0:32:33 |
| 2. | Alejandro Valverde (ESP, Caisse d'Epargne) | 0:08 |
| 3. | Maxime Monfort (BEL, Team HTC - Columbia) | 0:13 |
| 4. | Michael Rogers (AUS, Team HTC - Columbia) | 0:18 |
| 5. | Beñat Intxausti (ESP, Euskaltel - Euskadi) | 0:21 |
| 6. | Samuel Sánchez (ESP, Euskaltel - Euskadi) | 0:23 |
| 7. | Marco Pinotti (ITA, Team HTC - Columbia) | 0:25 |
| 8. | Joaquin Rodriguez (ESP, Team Katusha) | 0:33 |
| 9. | Jean-Christophe Peraud (FRA, Omega Pharma-Lotto) | 0:33 |
| 10. | Andreas Klöden (GER, Team Radioshack) | 0:34 |
| 11. | Peter Velits (SVK, Team HTC - Columbia) | 0:39 |
| 12. | Bradley Wiggins (GBR, Sky Professional Cycling Team) | 0:41 |
| 13. | Jacob Fuglsang (DEN, Team Saxo Bank) | 0:47 |
| 14. | Sandy Casar (FRA, Française Des Jeux) | 0:54 |
| 15. | Sergio Paulinho (POR, Team Radioshack) | 1:03 |
| 16. | Robert Gesink (NED, Rabobank) | 1:06 |
| 17. | Dries Devenyns (BEL, Quick Step) | 1:12 |
| 18. | Gustavo César (ESP, Xacobeo Galicia) | 1:14 |
| 19. | Ryder Hesjedal (CAN, Garmin - Transitions) | 1:21 |
| 20. | Jurgen Van Den Broeck (BEL, Omega Pharma-Lotto) | 1:22 |
| 21. | Haimar Zubeldia (ESP, Team Radioshack) | 1:23 |
| 22. | Jens Voigt (GER, Team Saxo Bank) | 1:23 |
| 23. | Thomas Löfkvist (SWE, Sky Professional Cycling Team) | 1:25 |
| 24. | Grischa Niermann (GER, Rabobank) | 1:29 |
| 25. | Daniel Martin (IRL, Garmin - Transitions) | 1:31 |
| 26. | Laurens Ten Dam (NED, Rabobank) | 1:37 |
| 27. | Vasili Kiryienka (BLR, Caisse d'Epargne) | 1:38 |
| 28. | David Zabriskie (USA, Garmin - Transitions) | 1:42 |
| 29. | Evgeny Petrov (RUS, Team Katusha) | 1:44 |
| 30. | Egoi Martínez (ESP, Euskaltel - Euskadi) | 1:48 |
| 31. | Davide Malacarne (ITA, Quick Step) | 1:52 |
| 32. | Eduard Vorganov (RUS, Team Katusha) | 1:58 |
| 33. | Bram Tankink (NED, Rabobank) | 1:58 |
| 34. | Jonas Aaen Jörgensen (DEN, Team Saxo Bank) | 2:04 |
| 35. | Ezequiel Mosquera (ESP, Xacobeo Galicia) | 2:04 |
| 36. | Valerio Agnoli (ITA, Liquigas-Doimo) | 2:06 |
| 37. | Alexandre Botcharov (RUS, Team Katusha) | 2:07 |
| 38. | Delio Fernandez (ESP, Xacobeo Galicia) | 2:10 |
| 39. | Juan Manuel Garate (ESP, Rabobank) | 2:10 |
| 40. | Dominik Nerz (GER, Team Milram) | 2:14 |
| 41. | Andy Schleck (LUX, Team Saxo Bank) | 2:14 |
| 42. | Charles Wegelius (GBR, Omega Pharma-Lotto) | 2:16 |
| 43. | Thomas Rohregger (AUT, Team Milram) | 2:21 |
| 44. | Jussi Veikkanen (FIN, Française Des Jeux) | 2:21 |
| 45. | Andrey Zeits (KAZ, Astana) | 2:21 |
| 46. | Daniel Navarro (ESP, Astana) | 2:21 |
| 47. | Serafín Martínez (ESP, Xacobeo Galicia) | 2:23 |
| 48. | Juan Jose Oroz (ESP, Euskaltel - Euskadi) | 2:23 |
| 49. | Xabier Zandio (ESP, Caisse d'Epargne) | 2:27 |
| 50. | Matthias Russ (GER, Team Milram) | 2:30 |
| 51. | Paolo Tiralongo (ITA, Astana) | 2:31 |
| 52. | Ben Swift (GBR, Sky Professional Cycling Team) | 2:35 |
| 53. | Johannes Frölingher (GER, Team Milram) |